Coding

Part:BBa_K3490000:Design

Designed by: Ren Hao Tsai   Group: iGEM20_NCKU_Tainan   (2020-10-23)


Nitric oxide synthase [Bacillus subtilis]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 151
    Illegal SapI.rc site found at 469


Design Notes

The strain of the bacteria needs to have a T7 promoter.


Source

Derived from Bacillus subtilis

References

‌BRENDA - Information on EC 1.14.13.39 - nitric-oxide synthase (NADPH). Brenda-enzymes.org. https://www.brenda-enzymes.org/enzyme.php?ecno=1.14.13.39#pH%20OPTIMUM. Published 2020. Accessed September 9, 2020.